Journal: EMBO Molecular Medicine

Article Title: GP64-pseudotyped lentiviral vectors target liver endothelial cells and correct hemophilia A mice

doi: 10.1038/s44321-024-00072-8

Figure Lengend Snippet: ( A , B ) Mean and SEM with single values of the % ( A ) or mean fluorescent intensity (MFI, B ) of GFP-positive primary human hepatocytes ( n = 3 technical replicates) transduced with VSV.G-LV or GP64-LV at the indicated multiplicity of infections (MOI). Mann–Whitney test. ( C ) Mean and SEM with single values of the amount of hFIX measured in the culture supernatant of primary human hepatocytes ( n = 3 technical replicates) transduced with VSV.G-LV or GP64-LV at the indicated MOI, or left untreated. ( D ). Mean and SEM with single values of the vector copy numbers (VCN) of reversed transcribed LV genome of the primary human hepatocytes (shown in ( A – C ), n = 3 technical replicates) transduced with VSV.G-LV or GP64-LV encoding for GFP or hFIX at the indicated MOI, or left untreated. ( E , F ) Mean and SEM with single values of the % ( E ) or MFI ( F ) of GFP-positive primary human immortalized LSEC derived from a healthy donor ( n = 4 independent experiments) transduced with VSV.G-LV or GP64-LV at the indicated MOI. ( G ) Mean and SEM with single values of the amount of hFVIII measured in the culture supernatant of primary human immortalized LSEC derived from a healthy donor ( n = 4 independent experiments) transduced with VSV.G-LV or GP64-LV at the indicated MOI, or left untreated. ( H ) Mean and SEM with single values of the VCN of reversed transcribed LV genome of the primary human LSEC (shown in ( E – G )) transduced with VSV.G-LV or GP64-LV encoding for GFP or hFVIII at the indicated MOI, or left untreated. .

Article Snippet: Immortalized human LSEC were purchased from Innoprot (P10652-IM).

Techniques: Transduction, MANN-WHITNEY, Plasmid Preparation, Derivative Assay